diff --git a/wp-admin/includes/ajax-actions.php b/wp-admin/includes/ajax-actions.php
index eca37d5e17..ec3b6f0f36 100644
--- a/wp-admin/includes/ajax-actions.php
+++ b/wp-admin/includes/ajax-actions.php
@@ -188,7 +188,12 @@ function wp_ajax_wp_compression_test() {
 	}
 
 	if ( ini_get( 'zlib.output_compression' ) || 'ob_gzhandler' === ini_get( 'output_handler' ) ) {
-		update_site_option( 'can_compress_scripts', 0 );
+		// Use `update_option()` on single site to mark the option for autoloading.
+		if ( is_multisite() ) {
+			update_site_option( 'can_compress_scripts', 0 );
+		} else {
+			update_option( 'can_compress_scripts', 0, 'yes' );
+		}
 		wp_die( 0 );
 	}
 
@@ -222,10 +227,20 @@ function wp_ajax_wp_compression_test() {
 			wp_die();
 		} elseif ( 'no' === $_GET['test'] ) {
 			check_ajax_referer( 'update_can_compress_scripts' );
-			update_site_option( 'can_compress_scripts', 0 );
+			// Use `update_option()` on single site to mark the option for autoloading.
+			if ( is_multisite() ) {
+				update_site_option( 'can_compress_scripts', 0 );
+			} else {
+				update_option( 'can_compress_scripts', 0, 'yes' );
+			}
 		} elseif ( 'yes' === $_GET['test'] ) {
 			check_ajax_referer( 'update_can_compress_scripts' );
-			update_site_option( 'can_compress_scripts', 1 );
+			// Use `update_option()` on single site to mark the option for autoloading.
+			if ( is_multisite() ) {
+				update_site_option( 'can_compress_scripts', 1 );
+			} else {
+				update_option( 'can_compress_scripts', 1, 'yes' );
+			}
 		}
 	}
 
diff --git a/wp-admin/includes/upgrade.php b/wp-admin/includes/upgrade.php
index 6959a4d1b3..9ef205ed0f 100644
--- a/wp-admin/includes/upgrade.php
+++ b/wp-admin/includes/upgrade.php
@@ -832,6 +832,10 @@ function upgrade_all() {
 		upgrade_600();
 	}
 
+	if ( $wp_current_db_version < 55853 ) {
+		upgrade_630();
+	}
+
 	maybe_disable_link_manager();
 
 	maybe_disable_automattic_widgets();
@@ -2293,6 +2297,29 @@ function upgrade_600() {
 	}
 }
 
+/**
+ * Executes changes made in WordPress 6.3.0.
+ *
+ * @ignore
+ * @since 6.3.0
+ *
+ * @global int $wp_current_db_version The old (current) database version.
+ */
+function upgrade_630() {
+	global $wp_current_db_version;
+
+	if ( $wp_current_db_version < 55853 ) {
+		if ( ! is_multisite() ) {
+			// Replace non-autoload option can_compress_scripts with autoload option, see #55270
+			$can_compress_scripts = get_option( 'can_compress_scripts', false );
+			if ( false !== $can_compress_scripts ) {
+				delete_option( 'can_compress_scripts' );
+				add_option( 'can_compress_scripts', $can_compress_scripts, 'yes' );
+			}
+		}
+	}
+}
+
 /**
  * Executes network-level upgrade routines.
  *
diff --git a/wp-includes/version.php b/wp-includes/version.php
index ae52e77d18..26f068a89c 100644
--- a/wp-includes/version.php
+++ b/wp-includes/version.php
@@ -16,14 +16,14 @@
  *
  * @global string $wp_version
  */
-$wp_version = '6.3-alpha-55853';
+$wp_version = '6.3-alpha-55854';
 
 /**
  * Holds the WordPress DB revision, increments when changes are made to the WordPress DB schema.
  *
  * @global int $wp_db_version
  */
-$wp_db_version = 53496;
+$wp_db_version = 55853;
 
 /**
  * Holds the TinyMCE version.