diff --git a/public/app/core/components/AppChrome/MegaMenu/MegaMenuHeader.tsx b/public/app/core/components/AppChrome/MegaMenu/MegaMenuHeader.tsx
index cfce7392d31..8d60cb8c904 100644
--- a/public/app/core/components/AppChrome/MegaMenu/MegaMenuHeader.tsx
+++ b/public/app/core/components/AppChrome/MegaMenu/MegaMenuHeader.tsx
@@ -23,7 +23,7 @@ export function MegaMenuHeader({ handleMegaMenu, handleDockedMenu, onClose }: Pr
 
   return (
     <div className={styles.header}>
-      <Stack alignItems="center" minWidth={0}>
+      <Stack alignItems="center" minWidth={0} gap={0.25}>
         <ToolbarButton narrow onClick={handleMegaMenu}>
           <Branding.MenuLogo className={styles.img} />
         </ToolbarButton>
@@ -69,7 +69,7 @@ const getStyles = (theme: GrafanaTheme2) => ({
     display: 'flex',
     gap: theme.spacing(1),
     justifyContent: 'space-between',
-    padding: theme.spacing(0, 1, 0, 0.5),
+    padding: theme.spacing(0, 1, 0, 0.75),
     height: TOP_BAR_LEVEL_HEIGHT,
     minHeight: TOP_BAR_LEVEL_HEIGHT,
   }),
diff --git a/public/app/core/components/AppChrome/MegaMenu/MegaMenuItem.tsx b/public/app/core/components/AppChrome/MegaMenu/MegaMenuItem.tsx
index 3640ff5fed9..d1e79208566 100644
--- a/public/app/core/components/AppChrome/MegaMenu/MegaMenuItem.tsx
+++ b/public/app/core/components/AppChrome/MegaMenu/MegaMenuItem.tsx
@@ -5,6 +5,7 @@ import { useLocation } from 'react-router-dom-v5-compat';
 import { useLocalStorage } from 'react-use';
 
 import { GrafanaTheme2, NavModelItem, toIconName } from '@grafana/data';
+import { config } from '@grafana/runtime';
 import { useStyles2, Text, IconButton, Icon, Stack } from '@grafana/ui';
 import { useGrafana } from 'app/core/context/GrafanaContext';
 
@@ -39,6 +40,7 @@ export function MegaMenuItem({ link, activeItem, level = 0, onClick, onPin, isPi
   );
   const showExpandButton = level < MAX_DEPTH && Boolean(linkHasChildren(link) || link.emptyMessage);
   const item = useRef<HTMLLIElement>(null);
+  const isSingleTopNav = config.featureToggles.singleTopNav;
 
   const styles = useStyles2(getStyles);
 
@@ -74,6 +76,29 @@ export function MegaMenuItem({ link, activeItem, level = 0, onClick, onPin, isPi
     );
   }
 
+  function getIconName(isExpanded: boolean) {
+    if (isSingleTopNav) {
+      return isExpanded ? 'angle-up' : 'angle-down';
+    } else {
+      return isExpanded ? 'angle-down' : 'angle-right';
+    }
+  }
+
+  const collapseIcon = (
+    <div className={styles.collapseButtonWrapper}>
+      {showExpandButton && (
+        <IconButton
+          aria-label={`${sectionExpanded ? 'Collapse' : 'Expand'} section ${link.text}`}
+          className={styles.collapseButton}
+          onClick={() => setSectionExpanded(!sectionExpanded)}
+          name={getIconName(Boolean(sectionExpanded))}
+          size="md"
+          variant="secondary"
+        />
+      )}
+    </div>
+  );
+
   return (
     <li ref={item} className={styles.listItem}>
       <div
@@ -83,18 +108,7 @@ export function MegaMenuItem({ link, activeItem, level = 0, onClick, onPin, isPi
       >
         {level !== 0 && <Indent level={level === MAX_DEPTH ? level - 1 : level} spacing={3} />}
         {level === MAX_DEPTH && <div className={styles.itemConnector} />}
-        <div className={styles.collapseButtonWrapper}>
-          {showExpandButton && (
-            <IconButton
-              aria-label={`${sectionExpanded ? 'Collapse' : 'Expand'} section ${link.text}`}
-              className={styles.collapseButton}
-              onClick={() => setSectionExpanded(!sectionExpanded)}
-              name={sectionExpanded ? 'angle-down' : 'angle-right'}
-              size="md"
-              variant="secondary"
-            />
-          )}
-        </div>
+        {!isSingleTopNav && collapseIcon}
         <div className={styles.collapsibleSectionWrapper}>
           <MegaMenuItemText
             isActive={isActive}
@@ -118,6 +132,7 @@ export function MegaMenuItem({ link, activeItem, level = 0, onClick, onPin, isPi
             </div>
           </MegaMenuItemText>
         </div>
+        {isSingleTopNav && collapseIcon}
       </div>
       {showExpandButton && sectionExpanded && (
         <ul className={styles.children}>
diff --git a/public/app/core/components/AppChrome/OrganizationSwitcher/OrganizationSwitcher.tsx b/public/app/core/components/AppChrome/OrganizationSwitcher/OrganizationSwitcher.tsx
index d9d75fb0b46..1fbcd63ca39 100644
--- a/public/app/core/components/AppChrome/OrganizationSwitcher/OrganizationSwitcher.tsx
+++ b/public/app/core/components/AppChrome/OrganizationSwitcher/OrganizationSwitcher.tsx
@@ -1,8 +1,9 @@
+import { css } from '@emotion/css';
 import { useEffect, useState } from 'react';
 
-import { SelectableValue } from '@grafana/data';
+import { GrafanaTheme2, SelectableValue } from '@grafana/data';
 import { config, locationService } from '@grafana/runtime';
-import { Text, useTheme2 } from '@grafana/ui';
+import { Text, useStyles2, useTheme2 } from '@grafana/ui';
 import { useMediaQueryChange } from 'app/core/hooks/useMediaQueryChange';
 import { contextSrv } from 'app/core/services/context_srv';
 import { getUserOrganizations, setUserOrganization } from 'app/features/org/state/actions';
@@ -17,6 +18,7 @@ export function OrganizationSwitcher() {
   const theme = useTheme2();
   const dispatch = useDispatch();
   const orgs = useSelector((state) => state.organization.userOrgs);
+  const styles = useStyles2(getStyles);
   const onSelectChange = (option: SelectableValue<UserOrg>) => {
     if (option.value) {
       setUserOrganization(option.value.orgId);
@@ -46,7 +48,11 @@ export function OrganizationSwitcher() {
 
   if (orgs?.length <= 1) {
     if (config.featureToggles.singleTopNav) {
-      return <Text truncate>{Branding.AppTitle}</Text>;
+      return (
+        <span className={styles.brandTitle}>
+          <Text truncate>{Branding.AppTitle}</Text>
+        </span>
+      );
     } else {
       return null;
     }
@@ -56,3 +62,9 @@ export function OrganizationSwitcher() {
 
   return <Switcher orgs={orgs} onSelectChange={onSelectChange} />;
 }
+
+const getStyles = (theme: GrafanaTheme2) => ({
+  brandTitle: css({
+    paddingLeft: theme.spacing(1),
+  }),
+});
diff --git a/public/app/core/components/AppChrome/TopBar/SingleTopBar.tsx b/public/app/core/components/AppChrome/TopBar/SingleTopBar.tsx
index 40db95e4000..de93562e32e 100644
--- a/public/app/core/components/AppChrome/TopBar/SingleTopBar.tsx
+++ b/public/app/core/components/AppChrome/TopBar/SingleTopBar.tsx
@@ -96,7 +96,7 @@ const getStyles = (theme: GrafanaTheme2, menuDockedAndOpen: boolean) => ({
     gap: theme.spacing(1),
     alignItems: 'center',
     padding: theme.spacing(0, 1),
-    paddingLeft: menuDockedAndOpen ? theme.spacing(3.5) : theme.spacing(0.5),
+    paddingLeft: menuDockedAndOpen ? theme.spacing(3.5) : theme.spacing(0.75),
     borderBottom: `1px solid ${theme.colors.border.weak}`,
     justifyContent: 'space-between',