diff --git a/packages/grafana-ui/src/components/IconButton/IconButton.story.tsx b/packages/grafana-ui/src/components/IconButton/IconButton.story.tsx
index db562ecb73b..0e410f60195 100644
--- a/packages/grafana-ui/src/components/IconButton/IconButton.story.tsx
+++ b/packages/grafana-ui/src/components/IconButton/IconButton.story.tsx
@@ -32,8 +32,6 @@ const meta: Meta<typeof IconButton> = {
     tooltip: 'sample tooltip message',
     tooltipPlacement: 'top',
     variant: 'secondary',
-    ariaLabel: 'this property is deprecated',
-    ['aria-label']: 'sample aria-label content',
   },
   argTypes: {
     tooltip: {
diff --git a/packages/grafana-ui/src/components/Tooltip/Tooltip.test.tsx b/packages/grafana-ui/src/components/Tooltip/Tooltip.test.tsx
index 12e7ece46d0..009b7e749f3 100644
--- a/packages/grafana-ui/src/components/Tooltip/Tooltip.test.tsx
+++ b/packages/grafana-ui/src/components/Tooltip/Tooltip.test.tsx
@@ -39,6 +39,7 @@ describe('Tooltip', () => {
 
     expect(refObj.current).not.toBeNull();
   });
+
   it('to be shown on hover and be dismissable by pressing Esc key when show is undefined', async () => {
     render(
       <Tooltip content="Tooltip content">
@@ -50,6 +51,7 @@ describe('Tooltip', () => {
     await userEvent.keyboard('{Escape}');
     expect(screen.queryByText('Tooltip content')).not.toBeInTheDocument();
   });
+
   it('is always visible when show prop is true', async () => {
     render(
       <Tooltip content="Tooltip content" show={true}>
@@ -61,6 +63,7 @@ describe('Tooltip', () => {
     await userEvent.unhover(screen.getByText('On the page'));
     expect(screen.getByText('Tooltip content')).toBeInTheDocument();
   });
+
   it('is never visible when show prop is false', async () => {
     render(
       <Tooltip content="Tooltip content" show={false}>
@@ -70,4 +73,27 @@ describe('Tooltip', () => {
     await userEvent.hover(screen.getByText('On the page'));
     expect(screen.queryByText('Tooltip content')).not.toBeInTheDocument();
   });
+
+  it('exposes the tooltip text to screen readers', async () => {
+    render(
+      <Tooltip content="Tooltip content">
+        <button>On the page</button>
+      </Tooltip>
+    );
+
+    // if tooltip is not visible, description won't be set
+    expect(
+      screen.queryByRole('button', {
+        description: 'Tooltip content',
+      })
+    ).not.toBeInTheDocument();
+
+    // tab to button to make tooltip visible
+    await userEvent.keyboard('{tab}');
+    expect(
+      await screen.findByRole('button', {
+        description: 'Tooltip content',
+      })
+    ).toBeInTheDocument();
+  });
 });
diff --git a/packages/grafana-ui/src/components/Tooltip/Tooltip.tsx b/packages/grafana-ui/src/components/Tooltip/Tooltip.tsx
index b3c78762360..0b881601f84 100644
--- a/packages/grafana-ui/src/components/Tooltip/Tooltip.tsx
+++ b/packages/grafana-ui/src/components/Tooltip/Tooltip.tsx
@@ -1,4 +1,4 @@
-import React, { useCallback, useEffect } from 'react';
+import React, { useCallback, useEffect, useId, useState } from 'react';
 import { usePopperTooltip } from 'react-popper-tooltip';
 
 import { GrafanaTheme2 } from '@grafana/data';
@@ -24,7 +24,8 @@ export interface TooltipProps {
 
 export const Tooltip = React.forwardRef<HTMLElement, TooltipProps>(
   ({ children, theme, interactive, show, placement, content }, forwardedRef) => {
-    const [controlledVisible, setControlledVisible] = React.useState(show);
+    const [controlledVisible, setControlledVisible] = useState(show);
+    const tooltipId = useId();
 
     useEffect(() => {
       if (controlledVisible !== false) {
@@ -44,10 +45,9 @@ export const Tooltip = React.forwardRef<HTMLElement, TooltipProps>(
 
     const { getArrowProps, getTooltipProps, setTooltipRef, setTriggerRef, visible, update } = usePopperTooltip({
       visible: show ?? controlledVisible,
-      placement: placement,
-      interactive: interactive,
+      placement,
+      interactive,
       delayHide: interactive ? 100 : 0,
-      delayShow: 150,
       offset: [0, 8],
       trigger: ['hover', 'focus'],
       onVisibleChange: setControlledVisible,
@@ -69,17 +69,23 @@ export const Tooltip = React.forwardRef<HTMLElement, TooltipProps>(
       [forwardedRef, setTriggerRef]
     );
 
+    // if the child has an aria-label, this should take precedence over the tooltip content
+    const childHasAriaLabel = 'aria-label' in children.props;
+
     return (
       <>
         {React.cloneElement(children, {
           ref: handleRef,
-          tabIndex: 0, // tooltip should be keyboard focusable
+          tabIndex: 0, // tooltip trigger should be keyboard focusable
+          'aria-describedby': !childHasAriaLabel && visible ? tooltipId : undefined,
         })}
         {visible && (
           <Portal>
             <div
               data-testid={selectors.components.Tooltip.container}
               ref={setTooltipRef}
+              id={tooltipId}
+              role="tooltip"
               {...getTooltipProps({ className: style.container })}
             >
               <div {...getArrowProps({ className: style.arrow })} />
diff --git a/public/app/plugins/datasource/azuremonitor/components/TracesQueryEditor/Filters.test.tsx b/public/app/plugins/datasource/azuremonitor/components/TracesQueryEditor/Filters.test.tsx
index 37676d0ee1a..7b15cfc923c 100644
--- a/public/app/plugins/datasource/azuremonitor/components/TracesQueryEditor/Filters.test.tsx
+++ b/public/app/plugins/datasource/azuremonitor/components/TracesQueryEditor/Filters.test.tsx
@@ -402,10 +402,8 @@ describe(`Traces Filters`, () => {
         ],
       },
     };
-    const removeLabel = screen.getAllByLabelText(`Remove`);
-    await act(async () => {
-      await userEvent.click(removeLabel[1]);
-    });
+    const removeLabel = screen.getAllByLabelText(/Remove/);
+    await userEvent.click(removeLabel[1]);
 
     rerender(
       <Filters
diff --git a/public/app/plugins/panel/annolist/AnnoListPanel.test.tsx b/public/app/plugins/panel/annolist/AnnoListPanel.test.tsx
index bf927c1de6d..f42c57ff7b7 100644
--- a/public/app/plugins/panel/annolist/AnnoListPanel.test.tsx
+++ b/public/app/plugins/panel/annolist/AnnoListPanel.test.tsx
@@ -269,7 +269,7 @@ describe('AnnoListPanel', () => {
           'anno-list-panel-1'
         );
         expect(screen.getByText(/filter:/i)).toBeInTheDocument();
-        expect(screen.getByText(/result email/i)).toBeInTheDocument();
+        expect(screen.getByRole('button', { name: /result email/i })).toBeInTheDocument();
       });
     });
 
diff --git a/public/app/plugins/panel/annolist/AnnotationListItem.tsx b/public/app/plugins/panel/annolist/AnnotationListItem.tsx
index 0e30c62fc35..a90fc53ce03 100644
--- a/public/app/plugins/panel/annolist/AnnotationListItem.tsx
+++ b/public/app/plugins/panel/annolist/AnnotationListItem.tsx
@@ -86,7 +86,7 @@ const Avatar = ({ onClick, avatarUrl, login, email }: AvatarProps) => {
 
   return (
     <Tooltip content={tooltipContent} theme="info" placement="top">
-      <button onClick={onAvatarClick} className={styles.avatar} aria-label={`Created by ${email}`}>
+      <button onClick={onAvatarClick} className={styles.avatar}>
         <img src={avatarUrl} alt="avatar icon" />
       </button>
     </Tooltip>