diff --git a/package.json b/package.json
index 4fd7096a41d..29419d9f4f5 100644
--- a/package.json
+++ b/package.json
@@ -256,7 +256,7 @@
     "@grafana/lezer-traceql": "0.0.11",
     "@grafana/monaco-logql": "^0.0.7",
     "@grafana/runtime": "workspace:*",
-    "@grafana/scenes": "^1.24.6",
+    "@grafana/scenes": "^1.26.0",
     "@grafana/schema": "workspace:*",
     "@grafana/ui": "workspace:*",
     "@kusto/monaco-kusto": "^7.4.0",
diff --git a/public/app/features/dashboard-scene/serialization/__snapshots__/transformSceneToSaveModel.test.ts.snap b/public/app/features/dashboard-scene/serialization/__snapshots__/transformSceneToSaveModel.test.ts.snap
index 8a3d5530c26..2295c3b3c0d 100644
--- a/public/app/features/dashboard-scene/serialization/__snapshots__/transformSceneToSaveModel.test.ts.snap
+++ b/public/app/features/dashboard-scene/serialization/__snapshots__/transformSceneToSaveModel.test.ts.snap
@@ -520,6 +520,7 @@ exports[`transformSceneToSaveModel Given a simple scene with custom settings Sho
           "text": "A",
           "value": "A",
         },
+        "definition": "*",
         "includeAll": false,
         "name": "query0",
         "options": [],
@@ -842,6 +843,7 @@ exports[`transformSceneToSaveModel Given a simple scene with variables Should tr
           "text": "A",
           "value": "A",
         },
+        "definition": "*",
         "includeAll": false,
         "name": "query0",
         "options": [],
diff --git a/public/app/features/dashboard-scene/serialization/sceneVariablesSetToVariables.test.ts b/public/app/features/dashboard-scene/serialization/sceneVariablesSetToVariables.test.ts
index 3528bc262f3..eba7dd50d8e 100644
--- a/public/app/features/dashboard-scene/serialization/sceneVariablesSetToVariables.test.ts
+++ b/public/app/features/dashboard-scene/serialization/sceneVariablesSetToVariables.test.ts
@@ -120,6 +120,58 @@ describe('sceneVariablesSetToVariables', () => {
         "type": "fake-std",
         "uid": "fake-std",
       },
+      "definition": undefined,
+      "description": "test-desc",
+      "includeAll": true,
+      "label": "test-label",
+      "multi": true,
+      "name": "test",
+      "options": [],
+      "query": "query",
+      "refresh": 1,
+      "regex": "",
+      "type": "query",
+    }
+    `);
+  });
+
+  it('should handle QueryVariable with definition set', () => {
+    const variable = new QueryVariable({
+      name: 'test',
+      label: 'test-label',
+      description: 'test-desc',
+      value: ['selected-value'],
+      text: ['selected-value-text'],
+      datasource: { uid: 'fake-std', type: 'fake-std' },
+      query: 'query',
+      definition: 'query',
+      includeAll: true,
+      allValue: 'test-all',
+      isMulti: true,
+    });
+    const set = new SceneVariableSet({
+      variables: [variable],
+    });
+
+    const result = sceneVariablesSetToVariables(set);
+
+    expect(result).toHaveLength(1);
+    expect(result[0]).toMatchInlineSnapshot(`
+    {
+      "allValue": "test-all",
+      "current": {
+        "text": [
+          "selected-value-text",
+        ],
+        "value": [
+          "selected-value",
+        ],
+      },
+      "datasource": {
+        "type": "fake-std",
+        "uid": "fake-std",
+      },
+      "definition": "query",
       "description": "test-desc",
       "includeAll": true,
       "label": "test-label",
diff --git a/public/app/features/dashboard-scene/serialization/sceneVariablesSetToVariables.ts b/public/app/features/dashboard-scene/serialization/sceneVariablesSetToVariables.ts
index 07cd8411531..535541827cb 100644
--- a/public/app/features/dashboard-scene/serialization/sceneVariablesSetToVariables.ts
+++ b/public/app/features/dashboard-scene/serialization/sceneVariablesSetToVariables.ts
@@ -25,6 +25,7 @@ export function sceneVariablesSetToVariables(set: SceneVariables) {
         },
         options: [],
         query: variable.state.query,
+        definition: variable.state.definition,
         datasource: variable.state.datasource,
         sort: variable.state.sort,
         refresh: variable.state.refresh,
diff --git a/public/app/features/dashboard-scene/serialization/transformSaveModelToScene.test.ts b/public/app/features/dashboard-scene/serialization/transformSaveModelToScene.test.ts
index a76035f3603..6ad41abaccc 100644
--- a/public/app/features/dashboard-scene/serialization/transformSaveModelToScene.test.ts
+++ b/public/app/features/dashboard-scene/serialization/transformSaveModelToScene.test.ts
@@ -455,7 +455,7 @@ describe('transformSaveModelToScene', () => {
       });
     });
 
-    it('should migrate query variable', () => {
+    it('should migrate query variable with definition', () => {
       const variable: QueryVariableModel = {
         allValue: null,
         current: {
@@ -467,7 +467,7 @@ describe('transformSaveModelToScene', () => {
           uid: 'P15396BDD62B2BE29',
           type: 'influxdb',
         },
-        definition: '',
+        definition: 'SHOW TAG VALUES  WITH KEY = "datacenter"',
         hide: 0,
         includeAll: false,
         label: 'Datacenter',
@@ -536,6 +536,7 @@ describe('transformSaveModelToScene', () => {
         type: 'query',
         value: 'America',
         hide: 0,
+        definition: 'SHOW TAG VALUES  WITH KEY = "datacenter"',
       });
     });
 
diff --git a/public/app/features/dashboard-scene/serialization/transformSaveModelToScene.ts b/public/app/features/dashboard-scene/serialization/transformSaveModelToScene.ts
index f67f1353f2e..8c0b20ed9a1 100644
--- a/public/app/features/dashboard-scene/serialization/transformSaveModelToScene.ts
+++ b/public/app/features/dashboard-scene/serialization/transformSaveModelToScene.ts
@@ -310,6 +310,7 @@ export function createSceneVariableFromVariableModel(variable: TypedVariableMode
       isMulti: variable.multi,
       skipUrlSync: variable.skipUrlSync,
       hide: variable.hide,
+      definition: variable.definition,
     });
   } else if (variable.type === 'datasource') {
     return new DataSourceVariable({
diff --git a/yarn.lock b/yarn.lock
index 0c26acb1821..fbdbdd6af26 100644
--- a/yarn.lock
+++ b/yarn.lock
@@ -3302,9 +3302,9 @@ __metadata:
   languageName: unknown
   linkType: soft
 
-"@grafana/scenes@npm:^1.24.6":
-  version: 1.24.6
-  resolution: "@grafana/scenes@npm:1.24.6"
+"@grafana/scenes@npm:^1.26.0":
+  version: 1.26.0
+  resolution: "@grafana/scenes@npm:1.26.0"
   dependencies:
     "@grafana/e2e-selectors": "npm:10.0.2"
     react-grid-layout: "npm:1.3.4"
@@ -3316,7 +3316,7 @@ __metadata:
     "@grafana/runtime": 10.0.3
     "@grafana/schema": 10.0.3
     "@grafana/ui": 10.0.3
-  checksum: 71df3f23f2a9083643ff2344960c01bcd87e7ff8c0802c8074e11d2b1acc9b99c0a6a3239c75db47b2507493beb83374dca4e02787b486d45f4dcbd5400a4055
+  checksum: a4d00396552ef048c28a51d4a4d36a7c9a9fb3ca193641002b279a33a5e85c10b1e63b859c0f42c6cc34992016fb18c10566817ac04c637dc48a0e85d21a93ab
   languageName: node
   linkType: hard
 
@@ -17332,7 +17332,7 @@ __metadata:
     "@grafana/lezer-traceql": "npm:0.0.11"
     "@grafana/monaco-logql": "npm:^0.0.7"
     "@grafana/runtime": "workspace:*"
-    "@grafana/scenes": "npm:^1.24.6"
+    "@grafana/scenes": "npm:^1.26.0"
     "@grafana/schema": "workspace:*"
     "@grafana/tsconfig": "npm:^1.3.0-rc1"
     "@grafana/ui": "workspace:*"