diff --git a/src/plugins/intel_gna/layers/gna_split_layer.hpp b/src/plugins/intel_gna/layers/gna_split_layer.hpp
index db3b01619a2..44edbe75f1a 100644
--- a/src/plugins/intel_gna/layers/gna_split_layer.hpp
+++ b/src/plugins/intel_gna/layers/gna_split_layer.hpp
@@ -50,7 +50,7 @@ public:
 // @brief Returns sizes of split outputs to split the input tensor to aligned parts not greater than the specified size
 static std::vector<uint32_t> GetAlignedSplitSizes(uint32_t totalSize, uint32_t maxSplitSize, uint32_t alignment = GNALimitations::inputByteAlignment) {
     std::vector<uint32_t> splitSizes;
-    uint32_t maxAlignedSplitSize = maxSplitSize - maxSplitSize % alignment;
+    uint32_t maxAlignedSplitSize = std::max(maxSplitSize - maxSplitSize % alignment, alignment);
     uint32_t usedSize = 0;
     while (usedSize < totalSize) {
         uint32_t partSize = std::min(totalSize - usedSize, maxAlignedSplitSize);
diff --git a/src/tests/unit/gna/ngraph/transformations/gna_split_eltwise.cpp b/src/tests/unit/gna/ngraph/transformations/gna_split_eltwise.cpp
index 9d63976e9bf..ae6a5c95742 100644
--- a/src/tests/unit/gna/ngraph/transformations/gna_split_eltwise.cpp
+++ b/src/tests/unit/gna/ngraph/transformations/gna_split_eltwise.cpp
@@ -149,7 +149,8 @@ const std::vector<ov::Shape> inputShape = {
     {1, 67000},
     {1, 500000},
     {1, 936, 513},
-    {1, 64, 64, 64}
+    {1, 64, 64, 64},
+    {1, 256, 64, 64}
 };
 
 INSTANTIATE_TEST_SUITE_P(SplitEltwiseTestSuite, SplitEltwiseTestSuiteFixture,