diff --git a/src/plugins/intel_gpu/src/graph/impls/ocl/resample.cpp b/src/plugins/intel_gpu/src/graph/impls/ocl/resample.cpp
index cac1e45d9e1..75e203380e9 100644
--- a/src/plugins/intel_gpu/src/graph/impls/ocl/resample.cpp
+++ b/src/plugins/intel_gpu/src/graph/impls/ocl/resample.cpp
@@ -212,6 +212,11 @@ attach_resample_impl::attach_resample_impl() {
         std::make_tuple(data_types::f16, format::bs_fs_yx_bsv32_fsv16),
         std::make_tuple(data_types::u8, format::bs_fs_yx_bsv32_fsv16),
         std::make_tuple(data_types::i8, format::bs_fs_yx_bsv32_fsv16),
+
+        std::make_tuple(data_types::f32, format::bs_fs_yx_bsv16_fsv16),
+        std::make_tuple(data_types::f16, format::bs_fs_yx_bsv16_fsv16),
+        std::make_tuple(data_types::u8, format::bs_fs_yx_bsv16_fsv16),
+        std::make_tuple(data_types::i8, format::bs_fs_yx_bsv16_fsv16),
     });
 }
 
diff --git a/src/plugins/intel_gpu/src/graph/program.cpp b/src/plugins/intel_gpu/src/graph/program.cpp
index 43f178735a3..f997bd1c7be 100644
--- a/src/plugins/intel_gpu/src/graph/program.cpp
+++ b/src/plugins/intel_gpu/src/graph/program.cpp
@@ -1459,7 +1459,8 @@ void program::set_layout_optimizer_attributes(layout_optimizer& lo) {
             prim.type() != cldnn::roi_align::type_id() &&
             prim.type() != cldnn::adaptive_pooling::type_id() &&
             prim.type() != cldnn::bucketize::type_id() &&
-            prim.type() != cldnn::roll::type_id()) {
+            prim.type() != cldnn::roll::type_id() &&
+            prim.type() != cldnn::resample::type_id()) {
             can_use_bs_fs_yx_bsv16_fsv16 = false;
         }
     }
diff --git a/src/plugins/intel_gpu/tests/test_cases/resample_gpu_test.cpp b/src/plugins/intel_gpu/tests/test_cases/resample_gpu_test.cpp
index 62401c5a32a..50990061fb1 100644
--- a/src/plugins/intel_gpu/tests/test_cases/resample_gpu_test.cpp
+++ b/src/plugins/intel_gpu/tests/test_cases/resample_gpu_test.cpp
@@ -1888,9 +1888,7 @@ struct resample_opt_random_test : testing::TestWithParam<resample_opt_random_tes
         topo_opt.add(reorder("res_to_bfyx", "to_output_type", format::bfyx, params.input_type));
 
         auto build_opts_opt = build_options();
-        build_opts_opt.set_option(build_option::outputs({"to_output_type", "res_to_bfyx"}));
-        // optimize_data is turned on to test cross-layout
-        build_opts_opt.set_option(build_option::optimize_data(true));
+        build_opts_opt.set_option(build_option::outputs({"resample_opt", "to_output_type", "res_to_bfyx"}));
 
         network net_opt(engine, topo_opt, build_opts_opt);
 
@@ -1900,7 +1898,9 @@ struct resample_opt_random_test : testing::TestWithParam<resample_opt_random_tes
         // first execution of opt
         auto result_opt = net_opt.execute();
         auto output_opt = result_opt.at("res_to_bfyx").get_memory();
-
+        if (!format::is_simple_data_format(params.in_format)) {
+            ASSERT_FALSE(format::is_simple_data_format(result_opt.at("resample_opt").get_memory()->get_layout().format));
+        }
         if (check_result == true) {
             // Check data_types
             if (params.input_type == data_types::f32) {
@@ -1931,10 +1931,12 @@ INSTANTIATE_TEST_SUITE_P(resample_opt_smoke_nearest,
                                 { data_types::i8,  {1, 128, 13, 13},  {1, 128, 26, 26},  1, resample::InterpolateOp::InterpolateMode::NEAREST, 1, format::b_fs_yx_fsv32, format::b_fs_yx_fsv32, {}, {}},
                                 { data_types::i8,  {1, 128, 13, 13},  {1, 128, 26, 26},  1, resample::InterpolateOp::InterpolateMode::NEAREST, 1, format::bs_fs_yx_bsv32_fsv16, format::bs_fs_yx_bsv32_fsv16, {}, {}},
                                 { data_types::i8,  {1, 128, 13, 13},  {1, 128, 26, 26},  1, resample::InterpolateOp::InterpolateMode::NEAREST, 1, format::bs_fs_yx_bsv32_fsv32, format::bs_fs_yx_bsv32_fsv32, {}, {}},
+                                { data_types::i8,  {1, 128, 13, 13},  {1, 128, 26, 26},  1, resample::InterpolateOp::InterpolateMode::NEAREST, 1, format::bs_fs_yx_bsv16_fsv16, format::bs_fs_yx_bsv16_fsv16, {}, {}},
                                 { data_types::f16, {1, 128, 13, 13},  {1, 128, 26, 26},  1, resample::InterpolateOp::InterpolateMode::NEAREST, 1, format::b_fs_yx_fsv16, format::b_fs_yx_fsv16, {}, {}},
                                 { data_types::f16, {1, 128, 13, 13},  {1, 128, 26, 26},  1, resample::InterpolateOp::InterpolateMode::NEAREST, 1, format::b_fs_yx_fsv32, format::b_fs_yx_fsv32, {}, {}},
                                 { data_types::f16, {1, 128, 13, 13},  {1, 128, 26, 26},  1, resample::InterpolateOp::InterpolateMode::NEAREST, 1, format::bs_fs_yx_bsv32_fsv16, format::bs_fs_yx_bsv32_fsv16, {}, {}},
                                 { data_types::f16, {1, 128, 13, 13},  {1, 128, 26, 26},  1, resample::InterpolateOp::InterpolateMode::NEAREST, 1, format::bs_fs_yx_bsv32_fsv32, format::bs_fs_yx_bsv32_fsv32, {}, {}},
+                                { data_types::f16, {1, 128, 13, 13},  {1, 128, 26, 26},  1, resample::InterpolateOp::InterpolateMode::NEAREST, 1, format::bs_fs_yx_bsv16_fsv16, format::bs_fs_yx_bsv16_fsv16, {}, {}},
                             }
                         ));
 
@@ -1946,6 +1948,7 @@ INSTANTIATE_TEST_SUITE_P(resample_opt_smoke_linear_onnx,
                                 { data_types::f16, {1, 128, 13, 13},  {1, 128, 26, 26},  1, resample::InterpolateOp::InterpolateMode::LINEAR_ONNX, 1, format::b_fs_yx_fsv32, format::b_fs_yx_fsv32, {}, {}},
                                 { data_types::f16, {1, 128, 13, 13},  {1, 128, 26, 26},  1, resample::InterpolateOp::InterpolateMode::LINEAR_ONNX, 1, format::bs_fs_yx_bsv32_fsv16, format::bs_fs_yx_bsv32_fsv16, {}, {}},
                                 { data_types::f16, {1, 128, 13, 13},  {1, 128, 26, 26},  1, resample::InterpolateOp::InterpolateMode::LINEAR_ONNX, 1, format::bs_fs_yx_bsv32_fsv32, format::bs_fs_yx_bsv32_fsv32, {}, {}},
+                                { data_types::f16, {1, 128, 13, 13},  {1, 128, 26, 26},  1, resample::InterpolateOp::InterpolateMode::LINEAR_ONNX, 1, format::bs_fs_yx_bsv16_fsv16, format::bs_fs_yx_bsv16_fsv16, {}, {}},
                                 { data_types::f16, {1, 128, 13, 13},  {1, 128, 26, 26},  1, resample::InterpolateOp::InterpolateMode::LINEAR_ONNX, 1, format::b_fs_yx_fsv16, format::b_fs_yx_fsv32, {}, {}},
                             }
                         ));