diff --git a/CHANGES b/CHANGES
index 2c05a81fa..a74447a48 100644
--- a/CHANGES
+++ b/CHANGES
@@ -21,6 +21,7 @@ Incompatible changes
 * #4389: output directory will be created after loading extensions
 * autodoc does not generate warnings messages to the generated document even if
   :confval:`keep_warnings` is True.  They are only emitted to stderr.
+* shebang line is removed from generated conf.py
 
 Deprecated
 ----------
diff --git a/sphinx/templates/quickstart/conf.py_t b/sphinx/templates/quickstart/conf.py_t
index a1c00f8c7..2583b9891 100644
--- a/sphinx/templates/quickstart/conf.py_t
+++ b/sphinx/templates/quickstart/conf.py_t
@@ -1,6 +1,3 @@
-{% if PY3 -%}
-#!/usr/bin/env python3
-{% endif -%}
 # -*- coding: utf-8 -*-
 #
 # {{ project }} documentation build configuration file, created by