diff --git a/ApplicationLibCode/ProjectDataModel/RimSummaryCalculationCollection.cpp b/ApplicationLibCode/ProjectDataModel/RimSummaryCalculationCollection.cpp
index 50e76eb289..0cf30ba7f6 100644
--- a/ApplicationLibCode/ProjectDataModel/RimSummaryCalculationCollection.cpp
+++ b/ApplicationLibCode/ProjectDataModel/RimSummaryCalculationCollection.cpp
@@ -51,7 +51,10 @@ RimSummaryCalculation* RimSummaryCalculationCollection::createCalculation() cons
 //--------------------------------------------------------------------------------------------------
 void RimSummaryCalculationCollection::updateDataDependingOnCalculations()
 {
-    if ( calculations().empty() ) return;
+    // One more update is required after the last calculation is deleted, to remove the data created by that calculation.
+    if ( calculations().empty() && !m_hasDataFromCalculations ) return;
+
+    m_hasDataFromCalculations = !calculations().empty();
 
     // Refresh data sources tree
     // Refresh meta data for all summary cases and rebuild AddressNodes in the summary tree
diff --git a/ApplicationLibCode/ProjectDataModel/RimSummaryCalculationCollection.h b/ApplicationLibCode/ProjectDataModel/RimSummaryCalculationCollection.h
index eea4c734c5..17fea26379 100644
--- a/ApplicationLibCode/ProjectDataModel/RimSummaryCalculationCollection.h
+++ b/ApplicationLibCode/ProjectDataModel/RimSummaryCalculationCollection.h
@@ -43,4 +43,8 @@ public:
 
 private:
     void updateDataDependingOnCalculations();
+
+private:
+    // True if the last update was performed with calculations present. Used to detect deletion of the last calculation.
+    bool m_hasDataFromCalculations = false;
 };
diff --git a/ApplicationLibCode/UnitTests/CMakeLists.txt b/ApplicationLibCode/UnitTests/CMakeLists.txt
index b539c1887e..6201244576 100644
--- a/ApplicationLibCode/UnitTests/CMakeLists.txt
+++ b/ApplicationLibCode/UnitTests/CMakeLists.txt
@@ -167,6 +167,7 @@ set(SOURCE_UNITTEST_FILES
     ${CMAKE_CURRENT_LIST_DIR}/RimMockSummaryCase-Test.cpp
     ${CMAKE_CURRENT_LIST_DIR}/RimSummaryCalculation-Test.cpp
     ${CMAKE_CURRENT_LIST_DIR}/RifMultipleSummaryReaders-Test.cpp
+    ${CMAKE_CURRENT_LIST_DIR}/RimSummaryCalculationCollection-Test.cpp
     ${CMAKE_CURRENT_LIST_DIR}/RiaConnectorTools-Test.cpp
     ${CMAKE_CURRENT_LIST_DIR}/RigWellTargetMappingTools-Test.cpp
     ${CMAKE_CURRENT_LIST_DIR}/RicWellPathExportMswGeometryPath-Test.cpp
diff --git a/ApplicationLibCode/UnitTests/RimMockSummaryCase.h b/ApplicationLibCode/UnitTests/RimMockSummaryCase.h
index 6544395f7a..353c8c7472 100644
--- a/ApplicationLibCode/UnitTests/RimMockSummaryCase.h
+++ b/ApplicationLibCode/UnitTests/RimMockSummaryCase.h
@@ -54,6 +54,9 @@ public:
 
     size_t keywordCount() const override { return m_allResultAddresses.size(); }
 
+    void refreshCalculatedAddresses() override { m_refreshCalculatedAddressesCount++; }
+    int  refreshCalculatedAddressesCount() const { return m_refreshCalculatedAddressesCount; }
+
 private:
     struct VectorData
     {
@@ -64,6 +67,7 @@ private:
 
     QString                                        m_name = "MockCase";
     std::map<RifEclipseSummaryAddress, VectorData> m_data;
+    int                                            m_refreshCalculatedAddressesCount = 0;
 };
 
 //--------------------------------------------------------------------------------------------------
diff --git a/ApplicationLibCode/UnitTests/RimSummaryCalculationCollection-Test.cpp b/ApplicationLibCode/UnitTests/RimSummaryCalculationCollection-Test.cpp
new file mode 100644
index 0000000000..6aa2a70649
--- /dev/null
+++ b/ApplicationLibCode/UnitTests/RimSummaryCalculationCollection-Test.cpp
@@ -0,0 +1,94 @@
+#include "gtest/gtest.h"
+
+#include "Summary/RiaSummaryTools.h"
+
+#include "RifEclipseSummaryAddress.h"
+
+#include "RimMockSummaryCase.h"
+#include "RimProject.h"
+#include "RimSummaryAddress.h"
+#include "RimSummaryCalculation.h"
+#include "RimSummaryCalculationCollection.h"
+#include "RimSummaryCalculationVariable.h"
+#include "RimSummaryCaseMainCollection.h"
+#include "RimSummaryEnsemble.h"
+
+#include <vector>
+
+namespace
+{
+//--------------------------------------------------------------------------------------------------
+/// Create a calculation producing a single field address
+//--------------------------------------------------------------------------------------------------
+RimSummaryCalculation* createFieldCalculation()
+{
+    auto* calculation = dynamic_cast<RimSummaryCalculation*>( RimProject::current()->calculationCollection()->addCalculation() );
+
+    calculation->setExpression( "MY_CALCULATION := x + 1" );
+    calculation->parseExpression();
+
+    auto* variable = dynamic_cast<RimSummaryCalculationVariable*>( calculation->variables()->at( 0 ) );
+
+    RimSummaryAddress address;
+    address.setAddress( RifEclipseSummaryAddress::fieldAddress( "FOPT" ) );
+    variable->setSummaryAddress( address );
+
+    return calculation;
+}
+} // namespace
+
+//--------------------------------------------------------------------------------------------------
+/// The summary case main collection is a shared global object, so every test must leave it empty to
+/// keep the tests order independent.
+//--------------------------------------------------------------------------------------------------
+class RimSummaryCalculationCollectionTest : public ::testing::Test
+{
+protected:
+    RimSummaryCaseMainCollection* mainCollection() const { return RiaSummaryTools::summaryCaseMainCollection(); }
+
+    void TearDown() override
+    {
+        for ( auto* ensemble : mainCollection()->summaryEnsembles() )
+        {
+            mainCollection()->removeEnsemble( ensemble );
+            delete ensemble;
+        }
+    }
+};
+
+//--------------------------------------------------------------------------------------------------
+/// The addresses of the realizations of an ensemble are not created up front, and the calculated addresses of the realizations must be
+/// refreshed both when a calculation is created and when the last calculation is deleted.
+///
+/// https://github.com/OPM/ResInsight/issues/14559
+//--------------------------------------------------------------------------------------------------
+TEST_F( RimSummaryCalculationCollectionTest, RefreshCalculatedAddressesForAllRealizations )
+{
+    std::vector<RimMockSummaryCase*> mockCases;
+    std::vector<RimSummaryCase*>     summaryCases;
+    for ( int realizationNumber = 0; realizationNumber < 3; realizationNumber++ )
+    {
+        auto* summaryCase = createMockCase( realizationNumber );
+        mockCases.push_back( dynamic_cast<RimMockSummaryCase*>( summaryCase ) );
+        summaryCases.push_back( summaryCase );
+    }
+
+    mainCollection()->addEnsemble( summaryCases, "Ensemble", true );
+
+    auto* calculation = createFieldCalculation();
+
+    std::vector<int> refreshCountAfterCreate;
+    for ( auto* mockCase : mockCases )
+    {
+        EXPECT_GT( mockCase->refreshCalculatedAddressesCount(), 0 );
+        refreshCountAfterCreate.push_back( mockCase->refreshCalculatedAddressesCount() );
+    }
+
+    RimProject::current()->calculationCollection()->deleteCalculation( calculation );
+
+    // Deleting the last calculation must refresh the addresses, to discard the addresses created by the calculation
+    for ( size_t i = 0; i < mockCases.size(); i++ )
+    {
+        EXPECT_GT( mockCases[i]->refreshCalculatedAddressesCount(), refreshCountAfterCreate[i] );
+    }
+}